Bernhard-Nocht Institute
Bernhard-Nocht Str. 74
D-20359 Hamburg
Germany

BNIoutS/BNoutAs: sense ATGAAT TAC CAA GTC AAT GGT TAC
antisense CAT AAC CAG TCG GTA CAG CTA C
FraGment lenGth 195 bp

BNIinS/BNIAs: sense GAA GCT ATT CGT CAC GTT CG
antisense CTGTAGAAA ATC CTA GCT GGA G
Fragment length 110 bp

SAR1s/SAR1as: sense CCT CTC TTG TTC TTG CTC GCA
antisense TAT AGT GAG CCG CCA CAC ATG
Fragment length 150 bp

Amplicon sequence (BNI-1, Bernhard-Nocht Institute, Hamburg, Germany)
TACCGTAGACTCATCTCTATGATGGGTTTCAAAATGAATTACCA
AGTCAATGGTTACCCTAATATGTTTATCACCCGCGAAGAAGCTAT
TCGTCACGTTCGTGCGTGGATTGGCTTTGATGTAGAGGGCTGTCAT
GCAACTAGAGATGCTGTGGGTACTAACCTACCTCTCCAGCTAGGA
TTTTCTACAGGTGTTAACTTAGTAGCTGTACCGACTGGTTATGTTGA
CACTGAAAATAACACAGAATTCACCAGAGTTAATGCAAAACCTCC
ACCAGGTGACCAGTTTAAACATCTTATACC

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Centers for Disease Control & Prevention
National Centers for Infectious Diseases,
Atlanta GA 30333
1600 Clifton Road, NE, MS-C12
United States

SARS-specific primers:
Cor-p-F2 (+) 5'CTAACATGCTTAGGATAATGG 3',
Cor-p-F3 (+) 5'GCCTCTCTTGTTCTTGCTCGC 3',
Cor-p-R1 (-) 5'CAGGTAAGCGTAAAACTCATC 3',
Product size:
Cor-p-F2/Cor-p-R1 :368 bp
Cor-p-F3/Cor-p-R1 :348 bp

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- SARS-CoV Specific RT-PCR Primers（PDF）

Government Virus Unit
9/F Public Health Laboratory Centre
382 NAm Cheong Street
Shek Kip Mei
Kowloon, Hong Kong SAR
Hong Kong - SAR China

COR-1,COR-2: sense 5' CAC CGT TTC TAC AGG TTA GCT AAC GA 3'

antisense 5' AAA TGT TTA CGC AGG TAA GCG TAA AA 3'

Product size: 310 bp

Queen Mary Hospital
The University of Hong Kong
University Pathology Building
Hong Kong - SAR China

HKU: sense 5' TACACACCTCAGCGTTG 3'

antisense 5' CACGAACGTGACGAAT 3'

Product size 182 bps